Graduate School

Basics in molecular biology and biotechnology

This course is part of the programme:
Molecular Genetics and Biotechnology graduate study programme (Third Level)

Objectives and competences

The main goal of the “Introductory Course”, is to provide one-week extensive initial training to First-year PhD students, to provide them basic background knowledge, at the beginning of the academic year, necessary to be introduced the various fields of research.

Different group leaders provide to students a presentation of their research activities and projects and students will be also introduced to research facilities running at UNG and ICGEB.

Prerequisites

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Content (Syllabus outline)

  • Background introductory lectures related to UNG and ICGEB research activities in the following topics: DNA replication, recombination and repair, RNA processing and micro RNA metabolism, regulation of gene expression, dynamic organization of nuclear structure and function, protein synthesis and interactions, protein structure, cell cycle and cancer, human molecular genetics, animal models of human disease, molecular genetics of yeast, molecular genetics of Drosophila, molecular genetics of bacteria, introduction to animal virology, molecular and cellular immunology, bioinformatics.
  • Tutorials in informatics, proteomics, microscopy, flow cytometry, analytical methods, safety procedures.
  • Presentation of group leaders and presentation of research projects
  • Presentation of institutional technical facilities
  • Recommended readings and further personalized tutorials according to specific student’s needs.

Intended learning outcomes

  • Students learn basic knowledge of molecular biology and biotechnology.
  • Students are introduced to technical facilities at ICGEB and UNG.
  • Students meet scientists and researcher community and learn different research interests at ICGEB and UNG.

Readings

  • G. Alberts, Johnson, Lewis, Raff; Roberts, Watson: Molecular Biology of the Cell, 5th Ed., Garland Science, 2008
  • Benjamin Lewin, GENES IX, © Copyright 2014 Jones and Bartlett Publishers http://biology.jbpub.com/book/genes/
  • Nature Reviews in Cell Biology http://www.nature.com/nrm/index.html
  • Nature Reviews in Genetics http://www.nature.com/nrg/index.html
  • Literature relevant for the course.

Assessment

Questionnaire 100%

Lecturer's references

Professor in Biochemistry and Molecular Biology at University of Ljubljana.

Bibliography:

1. Kočevar N, Grazio SF, Komel R. Two-dimensional gel electrophoresis of gastric tissue in an alkaline pH range. Proteomics. 2014 Feb;14(2-3):311-21.

2. Jovčevska I, Kočevar N, Komel R. Glioma and glioblastoma – how much do we (not) know? Mol Clin Oncol. 2013 Nov;1(6):935-941.

3. Hudler P, Gorsic M, Komel R. Proteomic strategies and challenges in tumor metastasis research. Clin Exp Metastasis. 2010 Aug;27(6):441-51.

4. Peternel S, Komel R. Isolation of biologically active nanomaterial (inclusion bodies) from bacterial cells. Microb Cell Fact. 2010 Sep 10;9:66. doi: 10.1186/1475-2859-9-66.

5. Hudler P, Gorsic M, Komel R. Proteomic strategies and challenges in tumor metastasis research. Clin Exp Metastasis. 2010 Aug;27(6):441-51.

University course code: 8MG001

Year of study: 1

Semester: 1

Course principal:

Lecturer:

ECTS: 3

Workload:

  • Lectures: 30 hours
  • Individual work: 60 hours

Course type: compulsory

Languages: english

Learning and teaching methods:
• lectures • presentation and visit of laboratories • technical tutorials • individual work • recommended reading and further personalised tutorials according to specific student’s needs.