School for Viticulture and Enology

Plant Physiology and Biotechnology

This course is part of the programme:
Bachelor's programme in Viticulture and Enology (1st level)

Objectives and competences

The goal of the plant physiology and biotechnology course is the joining of different fields in plant studies, to enable the students to have an extensive insight in how the plants work and response to the environment, with the putative applications of this knowledge in biotechnology.

Prerequisites

Knowledge of biology and microbiology.

The course is associated with following courses: Viticulture, Agriculture and the environment, Trace elements in soils and plants, Plant pathology, and partly by Ampelography.

Content (Syllabus outline)

LECTURES

  • metabolic pathways typical for plants
  • mineral nutrition
  • growth and development
  • physiology of symbiosis and parasitism
  • plant respond to biotic and abiotic stress
  • organization of prokaryotic and eukaryotic genome
  • regulation of gene expression in plants
  • application of knowledge of physiology and
  • biotechnology: plant tissue culture and genetic engineering

EXCERCISES

o plant mineral nutrition

o the influence of hormone on growth and development

o plant micropropagation

o plant transformation

o biotic and abiotic stress

Intended learning outcomes

Students will be acquainted with basic principles of plant physiology, plant genetics, and with the tissue culture and genetically modified plant technology.

Readings

Taiz L. IN Zeiger E. 2002. PLANT PHYSIOLOGY. Sinauer Associates, Sunderland, Massachusetts.

Vodnik D. 2012. OSNOVE FIZIOLOGIJE RASTLIN. Biotehniška fakulteta, Ljubljana.

Purohit S.S. 2003. AGRICULTURAL BIOTECHNOLOGY. Agrobios India, Jodhpur.

Raspor P. 1996. BIOTEHNOLOGIJA. BIA, Ljubljana.

Izročki predavanj / Lectures slides

Assessment

Excercises (attending tutorials and filled workbook) as a condition for the provision of colloquium and exam. The colloquium is merged with therory exam, which means that it is written at the same time (40 % tutorials/60 % lectures)

Lecturer's references

Doc.dr. Maruša Pompe Novak is an assistant professor in the field of Biotechnology, partly employed at the University of Nova Gorica, as a member of the Wine Research Centre and the National Institute of Biology, where the head of the center Planta.

Publications from last 5 years

1. BAEBLER, Špela, WITEK, Kamil, PETEK, Marko, STARE, Katja, TUŠEK-ŽNIDARIČ, Magda, POMPE NOVAK, Maruša, RENAUT, Jenny, SZAJKO, K., STRZELCZYK-ŻYTA, D., MARCZEWSKI, W., MORGIEWICZ, K., GRUDEN, Kristina, HENNIG, Jacek. Salicylic acid is an indispensable component of the Ny-1 resistance-gene-mediated response against Potato virus Y infection in potato. Journal of Experimental Botany, ISSN 0022-0957, 2014, vol. 65, iss. 4, str. 1095-1109, doi: 10.1093/jxb/ert447. [COBISS.SI-ID 3027535]

2. RUPAR, Matevž, KOGOVŠEK, Polona, POMPE NOVAK, Maruša, GUTIERREZ-AGUIRRE, Ion, DELAUNAY, Agnes, JACQUOT, Emmanuel, RAVNIKAR, Maja. Assessment of SNaPshot and single step RT-qPCR methods for discriminating Potato virus Y (PVY) subgroups. Journal of virological methods, ISSN 0166-0934. [Print ed.], 2013, vol. 189, issue 1, str. 93-100. http://dx.doi.org/10.1016/j.jviromet.2013.01.013, doi: 10.1016/j.jviromet.2013.01.013. [COBISS.SI-ID 2745423]

3. DOBNIK, David, BAEBLER, Špela, KOGOVŠEK, Polona, POMPE NOVAK, Maruša, ŠTEBIH, Dejan, PANTER, Gabriela, JANEŽ, Nikolaja, MORISSET, Dany, ŽEL, Jana, GRUDEN, Kristina. [Beta]-1,3-glucanase class III promotes spread of PVY[sup]NTN and improves in planta protein production. Plant biotechnology reports, ISSN 1863-5466, 2013, vol. 7, iss. 4, str. 547-555. http://download.springer.com/static/pdf/423/art%253A10.1007%252Fs11816-013-0300-5. pdf?auth66=1394360616_6fff3dc62122715f11b65ca83f349cc5&ext=.pdf, doi: 10.1007/s11816-013-0300-5. [COBISS.SI-ID 2897999]

4. PETEK, Marko, TURNŠEK, Neža, BUH GAŠPARIČ, Meti, POMPE NOVAK, Maruša, GRUDEN, Kristina, SLAPAR, Nina, POPOVIČ, Tatjana, ŠTRUKELJ, Borut, JONGSMA, Maarten Anthonie. A complex of genes involved in adaptation of Leptinotarsa decemlineata larvae to induced potato defense. Archives of insect biochemistry and physiology, ISSN 0739-4462, 2012, vol. 79, no. 3, str. 153-181. http://onlinelibrary.wiley.com/doi/10.1002/arch.21017/pdf. [COBISS.SI-ID 3232369]

5. KOGOVŠEK, Polona, KLADNIK, Aleš, MLAKAR, Jana, TUŠEK-ŽNIDARIČ, Magda, DERMASTIA, Marina, RAVNIKAR, Maja, POMPE NOVAK, Maruša. Distribution of Potato virus Y in potato plant organs, tissues and cells. Phytopathology, ISSN 0031-949X. [Print ed.], 2011, vol. 101, no. 11, str. 1292-1300. http://dx.doi.org/10.1094/PHYTO-01-11-0020, doi: 10.1094/PHYTO-01-11-0020. [COBISS.SI-ID 2401359]

6. BAR-DROR, Tal, DERMASTIA, Marina, KLADNIK, Aleš, TUŠEK-ŽNIDARIČ, Magda, POMPE NOVAK, Maruša, MEIR, Shimon, BURD, Shaul, PHILOSOPH-HADAS, Sonia, ORI, Naomi, SONEGO, Lilian, DICKMAN, Martin B., LERS, Amnon. Programmed cell death occurs asymmetrically during abscission in tomato. The Plant cell, ISSN 1040-4651. [Tiskana izd.], 2011, vol. 23, no. 11, str. 4146-4163, doi: 10.1105/tpc.111.092494. [COBISS.SI-ID 2469199]

7. BAEBLER, Špela, STARE, Katja, KOVAČ, Maja, BLEJEC, Andrej, PREZELJ, Nina, STARE, Tjaša, KOGOVŠEK, Polona, POMPE NOVAK, Maruša, ROSAHL, S., RAVNIKAR, Maja, GRUDEN, Kristina. Dynamics of responses in compatible potato – potato virus Y interaction are modulated by salicylic acid. PloS one, ISSN 1932-6203, 2011, vol. 6, issue 12, str. 1-12. http://dx.doi.org/10.1371/journal.pone.0029009, doi: 10.1371/journal.pone.0029009. [COBISS.SI-ID 2492751]

8. ŠKERGET, Katja, TALER-VERČIČ, Ajda, BAVDEK, Andrej, HODNIK, Vesna, ČERU, Slavko, TUŠEK-ŽNIDARIČ, Magda, POMPE NOVAK, Maruša, KOPITAR-JERALA, Nataša, TURK, Vito, ANDERLUH, Gregor, ŽEROVNIK, Eva, et al. Interaction between oligomers of stefin B and amyloid-beta in vitro and in cells. The Journal of biological chemistry, ISSN 0021-9258, 2010, vol. 285, no. 5, str. 3201-3210. http://dx.doi.org/10.1074/jbc.M109.024620, doi: 10.1074/jbc.M109.024620. [COBISS.SI-ID 2152783]

9. ČEPIN, Urška, GUTIERREZ-AGUIRRE, Ion, BALAŽIC, Leonida, POMPE NOVAK, Maruša, GRUDEN, Kristina, RAVNIKAR, Maja. A one-step reverse transcription real-time PCR assay for the detection and quantitation of Grapevine fanleaf virus. Journal of virological methods, ISSN 0166-0934. [Print ed.], 2010, vol. 170, issues 1-2, str. 47-56. http://dx.doi.org/10.1016/j.jviromet.2010.08.018, doi: 10.1016/j.jviromet.2010.08.018. [COBISS.SI-ID 2280783]

10. KOGOVŠEK, Polona, POMPE NOVAK, Maruša, BAEBLER, Špela, ROTTER, Ana, GOW, Lisa, GRUDEN, Kristina, FOSTER, Gary D., BOONHAM, Neil, RAVNIKAR, Maja. Aggressive and mild Potato virus Y isolates trigger different specific responses in susceptible potato plants. Plant Pathology, ISSN 0032-0862, 2010, vol. 59, issue 6, str. 1121-1132. http://dx.doi.org/10.1111/j.1365-3059.2010.02340.x, doi: 10.1111/j.1365-3059.2010.02340.x. [COBISS.SI-ID 2254415]

11. SEDMAK, Bojan, CARMELI, Shmuel, POMPE NOVAK, Maruša, TUŠEK-ŽNIDARIČ, Magda, GRACH-POGREBINSKY, Olga, ELERŠEK, Tina, ŽUŽEK, Monika C., BUBIK, Anja, FRANGEŽ, Robert. Cyanobacterial cytoskeleton immunostaining: the detection of cyanobacterial cell lysis induced by planktopeptin BL1125. Journal of plankton research, ISSN 0142-7873, 2009, vol. 31, no. 11, str. 1321-1330. http://dx.doi.org/10.1093/plankt/fbp076, doi: 10.1093/plankt/fbp076. [COBISS.SI-ID 2070607]

12. BAEBLER, Špela, KREČIČ STRES, Hana, ROTTER, Ana, KOGOVŠEK, Polona, CANKAR, Katarina, KOK, Esther, GRUDEN, Kristina, KOVAČ, Maja, ŽEL, Jana, POMPE NOVAK, Maruša, RAVNIKAR, Maja. PVY[supra]NTN elicits a diverse gene expression response in different potato genotypes in the first 12 h after inoculation. Molecular plant pathology, ISSN 1464-6722, 2009, vol. 10, no. 2, str. 263-275. http://dx.doi.org/10.1111/J.1364-3703.2008.00530.X, doi: 10.1111/J.1364-3703.2008.00530.X. [COBISS.SI-ID 1940815]

13. ŠKERGET, Katja, VILFAN, Andrej, POMPE NOVAK, Maruša, TURK, Vito, WALTHO, Jonathan P., TURK, Dušan, ŽEROVNIK, Eva. The mechanism of amyloid-fibril formation by stefin B : temperature and protein concentration dependence of the rates. Proteins, ISSN 0887-3585. [Print ed.], 2009, vol. 74, no. 2, str. 425-436. [COBISS.SI-ID 21880103]

University course code: 1VV103

Year of study: 2

Semester: 2

Course principal:

Lecturer:

Assistant:

ECTS: 5

Workload:

  • Lectures: 45 hours
  • Exercises: 15 hours
  • Individual work: 90 hours

Course type: lectures, laboratory practice sessions

Languages: slovene

Learning and teaching methods:
lectures, laboratory practice, students' independent work