School for Viticulture and Enology

Biochemistry

This course is part of the programme:
Bachelor's programme in Viticulture and Enology (1st level)

Objectives and competences

basics of

biochemical processes and molecular biology, focused primarily on plant and yeast cells. Through lectures and seminars the students will get theoretical background in the field of biochemistry and molecular biology and learn about the basic experimental techniques used in the field.

The acquired knowledge will enable the students to critically and objectively judge the novelties in modern biotechnological approaches in fermentation, genetically modified organisms, diagnostics of grapevine, and other related topics.

Prerequisites

Knowledge of general and organic chemistry and biology.

It is the basis for understanding the material in subjects where the student meets the molecular basis of biological processes. It relates to the subjects basics of microbiology, plant physiology, plant pathology and enological chemistry.

Content (Syllabus outline)

biological macromolecules: structure and function of proteins, lipids and sugars;

metabolism: metabolism of carbohydrates, lipids, aminoacids and nucleotides, photosynthesis, energy metabolism, secondary metabolism, regulation of metabolism, membranes and transport;

molecular biology: structure and function of DNA, replication, recombination, transcription, translation, basic techniques in molecular biology

cell biology and yeast genetics: structure of eukaryotic cell, specific features of plant and yeast cells, yeast genetics and genomics, nature and laboratory strains of yeast;

genetically modified organisms: g. m. yeast strains, g. m. plant varieties, basic techniques used in their design, examples of use of genetically modified organisms in oenology.

TUTORIALS

Short seminars with demonstration of practical cases- focused on application of biochemistry and molecular biology in oenology and viticulture.

Intended learning outcomes

Knowledge and understanding of the biochemical processes in plant and yeast cells on molecular level.

Readings

Pringle, J.R.; Broach, J.R.; Jones, E.W. 1992-1997. The Yeast Saccharomyces: Genome Dynamics, Protein Synthesis, and Energetics; Gene Expression; Cell Cycle and Cell Biology. Cold Spring Harbor Press: 826+792+1131 s.

Assessment

Seminar assignment is obligatory condition for written examination (seminar/exam = 30/70)

Lecturer's references

Assistant professor for molecular and cell biology

Izbrane publikacije zadnjih 5 let/Selected publications from the last 5 years

1. BRONIARCZYK, Justyna, BERGANT MARUŠIČ, Martina, GOŹDZICKA-JÓZEFIAK, Anna, BANKS, Lawrence. Human papillomavirus infection requires the TSG101 component of the ESCRT machinery. Virology, ISSN 0042-6822, 2014, vol. 460/461, str. 83-90, doi: 10.1016/j.virol.2014.05.005. [COBISS.SI-ID 3403515]

2. SCHÄFER, Georgia, KABANDA, Siti, VAN ROOYEN, Beverly, BERGANT MARUŠIČ, Martina, BANKS, Lawrence, IQBAL PARKER, M. The role of inflammation in HPV infection of the Oesophagus. BMC cancer, ISSN 1471-2407, 2013, vol. 13, no. 1, str. 1-11, doi: 10.1186/1471-2407-13-185. [COBISS.SI-ID 2739451]

3. BERGANT MARUŠIČ, Martina, BANKS, Lawrence. SNX17 facilitates infection with diverse papillomavirus types. Journal of virology, ISSN 0022-538X, 2013, vol. 87, no. 2, str. 1270-1273, doi: 10.1128/JVI.01991-12. [COBISS.SI-ID 2637307]

4. ŽVAB, Urška, BERGANT MARUŠIČ, Martina, LAVRENČIČ ŠTANGAR, Urška. Microplate-based assays for the evaluation of antibacterial effects of photocatalytic coatings. Applied microbiology and biotechnology, ISSN 0175-7598, 2012, vol. 96, issue 5, str. 1341-1351, doi: 10.1007/s00253-012-4458-5. [COBISS.SI-ID 2576379]

5. BERGANT MARUŠIČ, Martina, OZBUN, Michelle A., CAMPOS, Samuel K., MYERS, Michael P., BANKS, Lawrence. Human papillomavirus L2 facilitates viral escape from late endosomes via sorting nexin 17. Traffic, ISSN 1398-9219. Print ed., 2012, vol. 13, no. 3, str. 455-467. [COBISS.SI-ID 2162427]

6. BERGANT MARUŠIČ, Martina, MENCIN, Nina, LIČEN, Mia, BANKS, Lawrence, ŠTERLINKO, Helena. Modification of human papillomavirus minor capsid protein L2 by sumoylation. Journal of virology, ISSN 0022-538X, 2010, vol. 84, no. 21, str. 11585-11589. [COBISS.SI-ID 1743611]

7. GABRIJEL, Mateja, BERGANT MARUŠIČ, Martina, KREFT, Marko, JERAS, Matjaž, ZOREC, Robert. Fused late endocytic compartments and immunostimulatory capacity of dendritic-tumor cell hybridomas. The journal of membrane biology, ISSN 0022-2631, 2009, letn. 229, št. 1, str. 11-18, doi: 10.1007/s00232-009-9171-7. [COBISS.SI-ID 25624281]

8. ŽVAB, Urška, LAVRENČIČ ŠTANGAR, Urška, BERGANT MARUŠIČ, Martina. Methodologies for the analysis of antimicrobial effects of immobilized photocatalytic materials. Applied microbiology and biotechnology, ISSN 0175-7598, 2014, vol. 98, issue 5, str. 1925-1936, doi: 10.1007/s00253-013-5464-y. [COBISS.SI-ID 3079675]

9. PIM, David, BERGANT MARUŠIČ, Martina, BOON, Siaw S., GANTI, Ketaki, KRANJEC, Christian, MASSIMI, Paola, SUBBAIAH, Vanitha K., THOMAS, Miranda, TOMAIĆ, Vjekoslav, BANKS, Lawrence. Human papillomaviruses and the specificity of PDZ domain targeting. FEBS journal, ISSN 1742-464X, 2012, vol. 279, no. 19, str. 3530-3537. [COBISS.SI-ID 2597883]

10. ŠTERLINKO, Helena, BERGANT MARUŠIČ, Martina, BANKS, Lawrence. Human papillomavirus infection, cancer & therapy. The Indian journal of medical research, ISSN 0971-5916, 2009, vol. 130, no.3, str. 277-285. [COBISS.SI-ID 1391099]

University course code: 1VV201

Year of study: 2

Semester: 1

Course principal:

Lecturer:

Assistant:

ECTS: 5

Workload:

  • Lectures: 30 hours
  • Seminar: 15 hours
  • Individual work: 105 hours

Course type: lectures, tutorials

Languages: slovene

Learning and teaching methods:
lectures, tutorials, students' independent work