High-Resolution Nuclear Magnetic Resonance

This course is part of the programme
Doctoral study programme Physics

Objectives and competences

The main goal of the course is the introduction of students in the practical work with advanced experiments of high-resolution nuclear magnetic resonance (NMR), which is the most powerful method for determination of conformation and dynamics of molecules in solution. Students acquire knowledge about modern NMR approaches for studies of macromolecules and molecular complexes in solution.

Prerequisites

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Content

    • Multidimensional pulse sequences • Determination of three-dimensional structure of molecules and molecular complexes • Determination of inter-molecular interactions • Studies of conformational flexibility of molecules • Determination of dynamic properties of molecules and molecular systems • Rational drug design • Case studies: application of high-resolution NMR in biophysics, structural, organic and medicinal chemistry and molecular biology • Case studies: application of high-resolution NMR in pharmaceutical industry

Intended learning outcomes

Knowledge and understanding:

• of the broad capabilities of modern high-resolution NMR methods for resolving the research and development tasks in academia and industry
• of the potentials and limitations of modern NMR methods for studies of molecular structure and dynamics in solution.
• of the application of experimentally determined conformational and dynamic properties of molecules to unravel research problems.
• of the operation of the modern NMR equipment with high magnetic fields and cryogenic NMR probe technology.

Readings

    • C. P. Slichter: Principles of Magnetic Resonance, Springer-Verlag. Third Edition 1992. • J.Cavanagh, W.J. Fairbrother, A.G. Palmer III, M. Rance, N.J. Skelton: Protein NMR Spectroscopy, Elsevier, Second edition 2007 • O. Jardetzky, J.F. Lefevre: Dynamics and the Problem of Recognition in Biological Macromolecules, Nato ACI Series, Series A: Life Sciences Vol. 288, 1996 • Recent scientific literature.

Assessment

Individul project task and oral examination (50/50). A passing grade in the individual project task is required for admittance to the oral examination.

Lecturer's references

Associate professor of physics at the University of Nova Gorica.

Bibliography