Graduate School

Regenerative medicine and tissue engineering

This course is part of the programme:
Molecular Genetics and Biotechnology graduate study programme (Third Level)

Objectives and competences

  • Students acquire knowledge of regenerative medicine that develops methods for regenerating, repairing or replacing damaged or diseased cells, organs or tissues and which involves the production and use of therapeutic stem cells, tissue engineering, and artificial organs.
  • Students learn about the importance of an interdisciplinary approach in restoring or replacing damaged or diseased tissue or organs.
  • Students gain a critical attitude towards different therapeutic approaches in regenerative medicine, know their perspectives and limitations; possible ethical concerns.

Prerequisites

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Content (Syllabus outline)

Regenerative medicine (introduction, meaning, translation approaches)

Preparation of cell cultures from surgical resects; methods of isolating, multiplying and studying cells; the importance of appropriate in vitro conditions.

Functional and structural studies of cell cultures and cell surgical tissue

Biomaterials as carriers in tissue engineering

Stem cells, their properties and role, as well as perspectives and constraints

Totipotent, pluripotent and multipotent cells Induction of pluripotent stem cells

Differentiation of stem cells

Embryonic stem cells

Use of stem cells in regenerative medicine and tissue engineering

Stem cells in the eye

Use of stem cells in epithelial regeneration

Fibroblasts and their transformation

Ethical aspects in the field of regenerative medicin

Intended learning outcomes

Students will learn about the most recent advancements in regenerative medicine and understand principles that define the stem cell properties, differentiation and reprogramming, as well as therapeutic approaches in regenerative medicine.

Readings

- Molecular Biology of the Cell, Editors: Alberts et al., Sixth edition, 2014, Garland Science, New York

- Relevant literature in the field

Assessment

Attendance and active participation in class (20%), seminar (30%) and final exam (50%): Project / Seminar work is a midterm exam. • For the Seminar, participants can use all kinds of published sources of information, but not personal communications. • Seminar will be assessed according to: • Originality • The power of scientific arguments • Scientific regularity • Transparency of written explanation The final written examination serves to assess the understanding of the subject contents. The final exam is a written examination and consists of essay questions. The participants will solve the exams independently.

Lecturer's references

Doc. dr. Sofija Andjelić is assistant professor of Animal Physiology. She works at the University Medical Centre Ljubljana, Department of Ophthalmology. Her research is focused on human eye tissues, lens and cornea, for tissue engineering. She is investigating the functional and structural characteristics of the postoperative tissue and cultures, as well as Ca2+ signaling in cells in order to understand the connection between the Ca2+ homeostasis in individual cells, intercellular communication, pathology and potential for regeneration.

RECEK, Nina, ANDJELIĆ, Sofija, HOJNIK, Nataša, FILIPIČ, Gregor, LAZOVIĆ, Saša, VESEL, Alenka, PRIMC, Gregor, MOZETIČ, Miran, HAWLINA, Marko, PETROVSKI, Goran, CVELBAR, Uroš. Microplasma induced cell morphological changes and apoptosis of Ex Vivo cultured human anterior lens epithelial cells relevance to capsular opacification. PloS one, 2016, vol. 11, no. 11, str. 0165883-1-0165883-19

ANDJELIĆ, S, DRAŠLAR, K, LUMI, X, YAN, X, GRAW, J, FACSKÓ, A, HAWLINA, M, PETROVSKI, G. Morphological and proliferative studies on ex vivo cultured human anterior lens epithelial cells-relevance to capsular opacification. Acta ophthalmologica, 2015, 93 (6): e499-e506.

GOSAK, M, MARKOVIČ, R, FAJMUT, A, MARHL, M, HAWLINA, M, ANDJELIĆ*, S. The analysis of intracellular and intercellular calcium signaling in human anterior lens capsule epithelial cells with regard to different types and stages of the cataract. PloS one, 2015, 10 (12)

SZABÓ, Dóra Júlia, NOER, Agate, NAGYMIHÁLY, Richárd, JOSIFOVSKA, Natasha, ANDJELIĆ, Sofija, VERÉB, Zoltán, FACSKÓ, Andrea, MOE, Morten C., PETROVSKI, Goran. Long-term cultures of human cornea limbal explants form 3D structures ex vivo: implications for tissue engineering and clinical applications. PloS one, 2015, 10 (11).

ANDJELIĆ, Sofija, LUMI, Xhevat, VERÉB, Zoltán, JOSIFOVSKA, Natasha, FACSKÓ, Andrea, HAWLINA, Marko, PETROVSKI, Goran. A simple method for establishing adherent ex vivo explant cultures from human eye pathologies for use in subsequent calcium imaging and inflammatory studies. Journal of Immunology Research, 2014, 1-10.

University course code: 8MG024

Year of study: 1

Semester: 1-2

Course principal:

ECTS: 3

Workload:

  • Lectures: 20 hours
  • Seminar: 20 hours
  • Individual work: 50 hours

Course type: elective

Languages: english

Learning and teaching methods:
• students are expected to actively participate in lectures and seminars and to attend classes. lectures will be complemented by discussion of the relevant literature in the field, which should improve students' critical thinking and communication skills. • independent study of literature, presentation of results. • as part of the course, students will prepare individual projects in written form, which will be then presented orally to professors and other participants.